Proteintech, 82941-1-RR, FBXO32 Recombinant monoclonal antibody

Size: 20ul / 100ul The FBXO32 (82941-1-RR) by Proteintech is a Recombinant antibody targeting FBXO32 in IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 82941-1-RR targets FBXO32 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: C2C12 cells Positive FC (Intra) detected in: U-251 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag29394 Product name: Recombinant human FBXO32 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 50-120 aa of BC024030 Sequence: MWVYRMETILHWQQQLNNIQITRPAFKGLTFTDLPLCLQLNIMQRLSDGRDLVSLGQAAPDLHVLSEDRLL Predict reactive species Full Name: F-box protein 32 Calculated Molecular Weight: 355 aa, 42 kDa GenBank Accession Number: BC024030 Gene Symbol: FBXO32 Gene ID (NCBI): 114907 RRID: AB_3670684 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q969P5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.