Proteintech, 82966-2-RR, ARHGEF19 Recombinant monoclonal antibody

Size: 20ul / 100ul The ARHGEF19 (82966-2-RR) by Proteintech is a Recombinant antibody targeting ARHGEF19 in IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 82966-2-RR targets ARHGEF19 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: U2OS cells Positive FC (Intra) detected in: U-2 OS, HeLa cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Rho guanine nucleotide exchange factors (RHOGEFs) are proteins that could regulate Rho GTPases, which can be involved in a variety of physiological processes, such as survival, development, differentiation, and motility. ARHGEF19 also known as WGEF, is a member of the RhoGEF family. ARHGEF19 contains two identical functional domains, including the Dbl homology (DH) and pleckstrin homology (PH) domain(PMID:32993957). ARHGEF19 was highly expressed in human breast cancer(PMID: 34813497). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag11645 Product name: Recombinant human ARHGEF19 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 454-802 aa of BC040640 Sequence: VVLDHCPAFRRVYLPYVTNQAYQERTYQRLLLENPRFPGILARLEESPVCQRLPLTSFLILPFQRITRLKMLVENILKRTAQGSEDEDMATKAFNALKELVQECNASVQSMKRTEELIHLSKKIHFEGKIFPLISQARWLVRHGELVELAPLPAAPPAKLKLSSKAVYLHLFNDCLLLSRRKELGKFAVFVHAKMAELQVRDLSLKLQGIPGHVFLLQLLHGQHMKHQFLLRARTESEKQRWISALCPSSPQEDKEVISEGEDCPQVQCVRTYKALHPDELTLEKTDILSVRTWTSDGWLEGVRLADGEKGWVPQAYVEEISSLSARLRNLRENKRVTSATSKLGEAPV Predict reactive species Full Name: Rho guanine nucleotide exchange factor (GEF) 19 Calculated Molecular Weight: 802 aa, 89 kDa GenBank Accession Number: BC040640 Gene Symbol: ARHGEF19 Gene ID (NCBI): 128272 RRID: AB_3670712 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8IW93 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.