Proteintech, 83073-2-RR, LCMT2 Recombinant monoclonal antibody

Size: 20ul / 100ul The LCMT2 (83073-2-RR) by Proteintech is a Recombinant antibody targeting LCMT2 in IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83073-2-RR targets LCMT2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information LCMT2, also known as tRNA wybutosine-synthesizing protein 4 (TYW4), is an S-adenosylmethionine (SAM)-dependent enzyme that catalyzes the final step of yW biosynthesis, methylation and methoxycarbonylation.LCMT2 has a conserved leucine carboxyl methyltransferase (LCM) domain in its N-terminal region (PMID: 16642040). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag11145 Product name: Recombinant human LCMT2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 340-686 aa of BC015949 Sequence: VFPASVVSSEGQVPNLKRYGHASVFLSPDVILSAGGFGEQEGRHCRVSQFHLLSRDCDSEWKGSQIGSCGTGVQWDGRLYHTMTRLSESRVLVLGGRLSPVSPALGVLQLHFFKSEDNNTEDLKVTITKAGRKDDSTLCCWRHSTTEVSCQNQEYLFVYGGRSVVEPVLSDWHFLHVGTMAWVRIPVEGEVPEARHSHSACTWQGGALIAGGLGASEEPLNSVLFLRPISCGFLWESVDIQPPITPRYSHTAHVLNGKLLLVGGIWIHSSSFPGVTVINLTTGLSSEYQIDTTYVPWPLMLHNHTSILLPEEQQLLLLGGGGNCFSFGTYFNPHTVTLDLSSLSAGQ Predict reactive species Full Name: leucine carboxyl methyltransferase 2 Calculated Molecular Weight: aa, 76 kDa GenBank Accession Number: BC015949 Gene Symbol: LCMT2 Gene ID (NCBI): 9836 RRID: AB_3670792 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O60294 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.