{"product_id":"proteintech-83078-5-rr","title":"Proteintech, 83078-5-RR, p300 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe p300 (83078-5-RR) by Proteintech is a Recombinant antibody targeting p300 in IF\/ICC, FC (Intra), IP, ELISA, ChIP-qPCR applications with reactivity to human samples\n83078-5-RR targets p300 in WB, IF\/ICC, FC (Intra), IP, ELISA, ChIP-qPCR applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive IP detected in: HeLa cells\nPositive IF\/ICC detected in: A431 cells, HepG2 cells\nPositive FC (Intra) detected in: THP-1 cells\nPositive ChIP-qPCR detected in: K-562 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\np300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: E1A binding protein p300\nCalculated Molecular Weight: 264 kDa\nObserved Molecular Weight: 300 kDa\nGenBank Accession Number: NM_001429\nGene Symbol: EP300\nGene ID (NCBI): 2033\nRRID: AB_3670795\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q09472\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888851439785,"sku":"83078-5-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-83078-5-rr","provider":"Iright","version":"1.0","type":"link"}