{"product_id":"proteintech-83095-1-rr","title":"Proteintech, 83095-1-RR, Acetyl-Histone H4 (Lys12) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Acetyl-Histone H4 (Lys12) (83095-1-RR) by Proteintech is a Recombinant antibody targeting Acetyl-Histone H4 (Lys12) in WB, IHC, IF\/ICC, Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human, mouse, rat samples\n83095-1-RR targets Acetyl-Histone H4 (Lys12) in WB, IHC, IF\/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Trichostatin A treated NIH\/3T3 cells, HEK-293 cells,  HSC-T6 cells,  NIH\/3T3 cells\nPositive IHC detected in: mouse small intestine tissue, human placenta tissue,  mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: Caco-2 cells, Trichostatin A treated NIH\/3T3 cells\nPositive Dot Blot detected in: peptide\nPositive ChIP-qPCR detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:150-1:600\nDOT BLOT: DOT BLOT : 1:10-1:100\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHistone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: histone cluster 1, H4a\nObserved Molecular Weight: 12 kDa\nGenBank Accession Number: BC069654\nGene Symbol: HIST1H4A\nGene ID (NCBI): 8359\nRRID: AB_3670809\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P62805\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888348090537,"sku":"83095-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-83095-1-rr","provider":"Iright","version":"1.0","type":"link"}