Proteintech, 83132-7-RR, MTCO2 Recombinant monoclonal antibody

Size: 20ul / 100ul The MTCO2 (83132-7-RR) by Proteintech is a Recombinant antibody targeting MTCO2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human samples 83132-7-RR targets MTCO2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, 143B.TK.P0 cells, 143B cells, HeLa cells, U-251 cells, A549 cells, MDA-MB-231 cells Positive IP detected in: HeLa cells Positive IHC detected in: human ovarian cancer, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cytochrome c oxidase II Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 23-26 kDa GenBank Accession Number: YP_003024029 Gene Symbol: MTCO2 Gene ID (NCBI): 4513 RRID: AB_3670835 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P00403 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.