Proteintech, 83146-1-RR, TMEM165 Recombinant monoclonal antibody

Size: 20ul / 100ul The TMEM165 (83146-1-RR) by Proteintech is a Recombinant antibody targeting TMEM165 in IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83146-1-RR targets TMEM165 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information TMEM165 is a putative divalent cation:proton antiporter that exchanges calcium or manganese ions for protons across the Golgi membrane. It mediates the reversible transport of calcium or manganese to the Golgi lumen driven by the proton gradient and possibly the membrane potential generated by V-ATPase, and provides calcium or manganese cofactors to resident Golgi enzymes and contributes to the maintenance of an acidic luminal Golgi pH required for proper functioning of the secretory pathway (PMID:22683087, 23569283, 27008884, 32047108). TMEM165 has two isoforms, with molecular weights of 28 kDa and 35 kDa respectively. It is also subject to ubiquitination modification, which may cause an increase in its molecular weight. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag14362 Product name: Recombinant human TMEM165 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 61-177 aa of BC003545 Sequence: VSTVLFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLLNGPGDVETGTSITVPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHC Predict reactive species Full Name: transmembrane protein 165 Calculated Molecular Weight: 324 aa, 35 kDa GenBank Accession Number: BC003545 Gene Symbol: TMEM165 Gene ID (NCBI): 55858 RRID: AB_3670844 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9HC07 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.