Proteintech, 83167-5-RR, TGFB2/TGF-beta 2 Recombinant monoclonal antibody

Size: 20ul / 100ul The TGFB2/TGF-beta 2 (83167-5-RR) by Proteintech is a Recombinant antibody targeting TGFB2/TGF-beta 2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83167-5-RR targets TGFB2/TGF-beta 2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: A549 cells, U-87 MG cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:125-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: transforming growth factor, beta 2 Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 48 kDa GenBank Accession Number: NM_003238 Gene Symbol: TGFB2 Gene ID (NCBI): 7042 RRID: AB_3670862 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P61812 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.