Proteintech, 83328-2-RR, VPS35 Recombinant monoclonal antibody

Size: 20ul / 100ul The VPS35 (83328-2-RR) by Proteintech is a Recombinant antibody targeting VPS35 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 83328-2-RR targets VPS35 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HepG2 cells, Raji cells, HEK-293 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information VPS35 protein belongs to a group of vacuolar protein sorting (VPS) proteins, which ensure the proper delivery of organelle-specific proteins in eukaryotic cells. VPS35 is the core of a multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. Vps35 serves as the core of the multimeric complex by binding directly to Vps26 and Vps29 and SNX1. Northern blot analyses in 16 tissues showed that one transcript of Vps35 with a size of 3.6 kb was highly expressed in brain, heart, testis, ovary, small intestine, spleen, skeletal muscle, and placenta and expressed at moderate or low levels in other tissues. Another transcript of Vps35, a message of 3.0 kb, was also expressed with proportionally lower levels than the 3.6-kb transcript in all the tissues except that the 3.0-kb transcript was not detected in brain. Human Vps35 is mapped at 16q13-q21. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0333 Product name: Recombinant human VPS35 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC002414 Sequence: MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQ Predict reactive species Full Name: vacuolar protein sorting 35 homolog (S. cerevisiae) Calculated Molecular Weight: 92 kDa Observed Molecular Weight: 80-92 kDa GenBank Accession Number: BC002414 Gene Symbol: VPS35 Gene ID (NCBI): 55737 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96QK1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.