{"product_id":"proteintech-83332-3-rr","title":"Proteintech, 83332-3-RR, Phospho-HSP27 (Ser15) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-HSP27 (Ser15) (83332-3-RR) by Proteintech is a Recombinant antibody targeting Phospho-HSP27 (Ser15) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n83332-3-RR targets Phospho-HSP27 (Ser15) in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Anisomycin treated HeLa cells\nPositive IF\/ICC detected in: Anisomycin treated HeLa cells\nPositive FC (Intra) detected in: Anisomycin treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: heat shock 27kDa protein 1\nCalculated Molecular Weight: 19-23 kDa\nObserved Molecular Weight: 27 kDa\nGenBank Accession Number: BC012768\nGene Symbol: HSPB1\nGene ID (NCBI): 3315\nRRID: AB_3670996\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P04792\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888439021737,"sku":"83332-3-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-83332-3-rr","provider":"Iright","version":"1.0","type":"link"}