{"product_id":"proteintech-83342-1-rr","title":"Proteintech, 83342-1-RR, RHOBTB1 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe RHOBTB1 (83342-1-RR) by Proteintech is a Recombinant antibody targeting RHOBTB1 in WB, ELISA applications with reactivity to Human samples\n83342-1-RR targets RHOBTB1 in WB, ELISA applications and shows reactivity with Human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HepG2 cells,  U-251 cells,  HT-29 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRho-related BTB domain containing protein 1 (RhoBTB1) is also named as KIAA0740. RhoBTB1 is an atypical Rho GTPase with two BTB domains in addition to its Rho domain. RhoBTB1 is a substrate for ROCK1, and mutation of putative phosphorylation sites reduces its association with Cullin3, a scaffold for ubiquitin ligases (PMID: 31431478). RHOBTB1 is a novel PPARγ gene target in vascular smooth muscle cells that mediates the protective effect of PPARγ by serving as a substrate adaptor between the Cullin-3 RING ubiquitin ligase and phosphodiesterase 5, thus restraining its activity through ubiquitination and proteasomal degradation (PMID: 31789920).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag3365 Product name: Recombinant human RHOBTB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-344 aa of BC032848 Sequence: MDADMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKSMWYPEIKHFCPRTPVILVGCQLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKELGLPYYETSVFDQFGIKDVFDNAIRAALISRRHLQFWKSHLKKVQKPLLQAPFLPPKAPPPVIKIPECPSMGTNEAACLLDNPLCADVLFILQDQEHIFAHRIYLATSSSKFYDLFLMECEESPNGSEGACEKEKQSRDFQGRILSVDPEEEREEGPPRIPQAD Predict reactive species\nFull Name: Rho-related BTB domain containing 1\nCalculated Molecular Weight: 696 aa, 79 kDa\nObserved Molecular Weight: 79 kDa\nGenBank Accession Number: BC032848\nGene Symbol: RHOBTB1\nGene ID (NCBI): 9886\nRRID: AB_3671003\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: O94844\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888660730025,"sku":"83342-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-83342-1-rr","provider":"Iright","version":"1.0","type":"link"}