Proteintech, 83540-1-RR, MED12L Recombinant monoclonal antibody

Size: 20ul / 100ul The MED12L (83540-1-RR) by Proteintech is a Recombinant antibody targeting MED12L in IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 83540-1-RR targets MED12L in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: U2OS cells, HepG2 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This antibody can recognize all the isoform of MED12L. This antibody has no cross-reaction to MED12. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag34636 Product name: Recombinant human MED12L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 627-706 aa of NM_053002 Sequence: LSVTASTRPRSPVGENADEHYSKDHDVKMEIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQ Predict reactive species Full Name: mediator complex subunit 12-like Calculated Molecular Weight: 240 kDa GenBank Accession Number: NM_053002 Gene Symbol: MED12L Gene ID (NCBI): 116931 RRID: AB_3671161 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q86YW9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.