Proteintech, 83590-5-RR, INTS4 Recombinant monoclonal antibody

Size: 20ul / 100ul The INTS4 (83590-5-RR) by Proteintech is a Recombinant antibody targeting INTS4 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83590-5-RR targets INTS4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells Positive IF/ICC detected in: U2OS cells, HeLa cells Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag9116 Product name: Recombinant human INTS4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-331 aa of BC009995 Sequence: MAAHLKKRVYEEFTKVVQPQEEIATKKLRLTKPSKSAALHIDLCKATSPADALQYLLQFARKPVEAESVEGVVRILLEHYYKENDPSVRLKIASLLGLLSKTAGFSPDCIMDDAINILQNEKSHQVLAQLLDTLLAIGTKLPENQAIQMRLVDVACKHLTDTSHGVRNKCLQLLGNLGSLEKSVTKDAEGLAARDVQKIIGDYFSDQDPRVRTAAIKAMLQLHERGLKLHQTIYNQACKLLSDDYEQVRSAAVQLIWVVSQLYPESIVPIPSSNEEIRLVDDAFGKICHMVSDGSWVVRVQAAKLLGSMEQVSSHFLEQTLDKKLMSDLRR Predict reactive species Full Name: integrator complex subunit 4 Calculated Molecular Weight: 963aa,108 kDa; 505aa,57 kDa Observed Molecular Weight: 108 kDa GenBank Accession Number: BC009995 Gene Symbol: INTS4 Gene ID (NCBI): 92105 RRID: AB_3671204 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96HW7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.