{"product_id":"proteintech-83591-2-rr","title":"Proteintech, 83591-2-RR, Drebrin Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Drebrin (83591-2-RR) by Proteintech is a Recombinant antibody targeting Drebrin in WB, IHC, IF\/ICC, IF-P, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n83591-2-RR targets Drebrin in WB, IHC, IF\/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, K-562 cells,  Neuro-2a cells,  SH-SY5Y cells,  PC-12 cells\nPositive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse brain tissue\nPositive IF\/ICC detected in: MCF-7 cells\nPositive FC (Intra) detected in: SH-SY5Y cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDrebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag0371 Product name: Recombinant human Drebrin protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 118-302 aa of BC000283 Sequence: ASSVEDIDAGAIGQRLSNGLARLSSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDERLRFEQERMEQERQEQEERERRYREREQQIEEHRRKQQTLEAEEAKRRLKEQSIFGDHRDEEEETHMKKSESEVEEAAAIIAQRPDNPREFFKQQERVAS Predict reactive species\nFull Name: drebrin 1\nCalculated Molecular Weight: 71 kDa\nObserved Molecular Weight: 110-130 kDa\nGenBank Accession Number: BC000283\nGene Symbol: Drebrin\nGene ID (NCBI): 1627\nENSEMBL Gene ID: ENSG00000113758\nRRID: AB_3671206\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q16643\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888420245673,"sku":"83591-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-83591-2-rr","provider":"Iright","version":"1.0","type":"link"}