Proteintech, 83708-1-RR, RAP1A Recombinant monoclonal antibody

Size: 20ul / 100ul The RAP1A (83708-1-RR) by Proteintech is a Recombinant antibody targeting RAP1A in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 83708-1-RR targets RAP1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, A431 cells, K-562 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, human placenta tissue Positive IF/ICC detected in: NIH/3T3 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag9488 Product name: Recombinant human RAP1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC014086 Sequence: MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL Predict reactive species Full Name: RAP1A, member of RAS oncogene family Calculated Molecular Weight: 184 aa, 21 kDa GenBank Accession Number: BC014086 Gene Symbol: RAP1A Gene ID (NCBI): 5906 RRID: AB_3671310 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P62834 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.