{"product_id":"proteintech-83920-6-rr","title":"Proteintech, 83920-6-RR, BUB1B Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe BUB1B (83920-6-RR) by Proteintech is a Recombinant antibody targeting BUB1B in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n83920-6-RR targets BUB1B in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells\nPositive IHC detected in: mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nBUB1B(Mitotic checkpoint serine\/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3, and SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID: 22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications, including acetylation(PMID: 22167194). Defects in BUB1B are the cause of the premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1). Research has shown that a BUB1B isoform with a measured MW of about 65 kDa (PMID: 34620840).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag34182 Product name: Recombinant human BUB1B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 719-1050 aa of BC018739 Sequence: QSPWCSQYRRQLLKSLPELSASAELCIEDRPMPKLEIEKEIELGNEDYCIKREYLICEDYKLFWVAPRNSAELTVIKVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFVRILNANDEATVSVLGELAAEMNGVFDTTFQSHLNKALWKVGKLTSPGALLFQ Predict reactive species\nFull Name: budding uninhibited by benzimidazoles 1 homolog beta (yeast)\nCalculated Molecular Weight: 1050 aa, 120 kDa\nObserved Molecular Weight: 130 kDa\nGenBank Accession Number: BC018739\nGene Symbol: BubR1\nGene ID (NCBI): 701\nRRID: AB_3671500\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: O60566\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888396751017,"sku":"83920-6-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-83920-6-rr","provider":"Iright","version":"1.0","type":"link"}