Proteintech, 83981-4-RR, Securin Recombinant monoclonal antibody

Size: 20ul / 100ul The Securin (83981-4-RR) by Proteintech is a Recombinant antibody targeting Securin in IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83981-4-RR targets Securin in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: Jurkat cells, A431 cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag12670 Product name: Recombinant human PTTG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-202 aa of BC101834 Sequence: MATLIYVDKENGEPGTRVVAKDGLKLGSGPSIKALDGRSQVSTPRFGKTFDAPPALPKATRKALGTVNRATEKSVKTKGPLKQKQPSFSAKKMTEKTVKAKSSVPASDDAYPEIEKFFPFNPLDFESFDLPEEHQIAHLPLSGVPLMILDEERELEKLFQLGPPSPVKMPSPPWESNLLQSPSSILSTLDVELPPVCCDIDI Predict reactive species Full Name: pituitary tumor-transforming 1 Calculated Molecular Weight: 202 aa, 22 kDa GenBank Accession Number: BC101834 Gene Symbol: Securin Gene ID (NCBI): 9232 RRID: AB_3671555 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O95997 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.