Proteintech, 84041-2-RR, PRC1 Recombinant monoclonal antibody

Size: 20ul / 100ul The PRC1 (84041-2-RR) by Proteintech is a Recombinant antibody targeting PRC1 in WB, IF/ICC, ELISA applications with reactivity to human samples 84041-2-RR targets PRC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, L02 cells, HeLa cells, HEK-293 cells, HCT 116 cells, C6 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag8028 Product name: Recombinant human PRC1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 154-446 aa of BC003138 Sequence: NQFRQHVTTLRETKASRREEFVSIKRQIILCMEELDHTPDTSFERDVVCEDEDAFCLSLENIATLQKLLRQLEMQKSQNEAVCEGLRTQIRELWDRLQIPEEEREAVATIMSGSKAKVRKALQLEVDRLEELKMQNMKKVIEAIRVELVQYWDQCFYSQEQRQAFAPFCAEDYTESLLQLHDAEIVRLKNYYEVHKELFEGVQKWEETWRLFLEFERKASDPNRFTNRGGNLLKEEKQRAKLQKMLPKLEEELKARIELWEQEHSKAFMVNGQKFMEYVAEQWEMHRLEKERA Predict reactive species Full Name: protein regulator of cytokinesis 1 Calculated Molecular Weight: 72 kDa Observed Molecular Weight: 66-72 kDa GenBank Accession Number: BC003138 Gene Symbol: PRC1 Gene ID (NCBI): 9055 RRID: AB_3671608 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O43663 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.