Proteintech, 84115-1-RR, PPID Recombinant monoclonal antibody

Size: 20ul / 100ul The PPID (84115-1-RR) by Proteintech is a Recombinant antibody targeting PPID in WB, FC (Intra), ELISA applications with reactivity to human samples 84115-1-RR targets PPID in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, MCF7 cells, Jurkat cells Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3409 Product name: Recombinant human PPID protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 57-370 aa of BC030707 Sequence: GIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA Predict reactive species Full Name: peptidylprolyl isomerase D Calculated Molecular Weight: 370 aa, 41 kDa Observed Molecular Weight: 41 kDa GenBank Accession Number: BC030707 Gene Symbol: PPID Gene ID (NCBI): 5481 RRID: AB_3671679 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q08752 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.