{"product_id":"proteintech-84135-4-rr","title":"Proteintech, 84135-4-RR, NELFB Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe NELFB (84135-4-RR) by Proteintech is a Recombinant antibody targeting NELFB in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n84135-4-RR targets NELFB in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HepG2 cells,  MCF-7 cells,  BxPC-3 cells,  A431 cells\nPositive IF\/ICC detected in: A431 cells\nPositive FC (Intra) detected in: A431 cells, BxPC-3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag9599 Product name: Recombinant human NELF-B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 127-475 aa of BC011892 Sequence: VHYCTLRAELLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVFKEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALEDFCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPAQAAETPALELPLPSVPAPAPL Predict reactive species\nFull Name: cofactor of BRCA1\nCalculated Molecular Weight: 580aa,66 kDa; 475aa,54 kDa\nObserved Molecular Weight: 66 kDa\nGenBank Accession Number: BC011892\nGene Symbol: NELFB\nGene ID (NCBI): 25920\nRRID: AB_3671694\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q8WX92\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888789737641,"sku":"84135-4-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-84135-4-rr","provider":"Iright","version":"1.0","type":"link"}