Proteintech, 84317-7-RR, SEL1L Recombinant monoclonal antibody

Size: 20ul / 100ul The SEL1L (84317-7-RR) by Proteintech is a Recombinant antibody targeting SEL1L in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 84317-7-RR targets SEL1L in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: NIH/3T3 cells, HepG2 cells, MCF-7 cells, BxPC-3 cells, U-87 MG cells, mouse kidney tissue, rat liver tissue Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:6000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information SEL1L (Suppressor Enhancer Lin-12 Like) is an endoplasmic reticulum-resident transmembrane protein that plays a critical role in collagen metabolism. It has been identified as an intracellular rheostat controlling collagen turnover, which is the balance between collagen synthesis and degradation. The protein's luminal face contains a Fibronectin-2 (FN2) domain, which is known to bind collagen. SEL1L functions as a sensor of collagen biosynthesis and is necessary for the upregulation of MRC2 (mannose receptor, C-type 2), a collagen endocytic receptor that mediates the clearance of collagen. . Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag32178 Product name: Recombinant human SEL1L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 22-225 aa of NM_005065 Sequence: SSDEEGSQDESLDSKTTLTSDESVKDHTTAGRVVAGQIFLDSEESELESSIQEEEDSLKSQEGESVTEDISFLESPNPENKDYEEPKKVRKPALTAIEGTAHGEPCHFPFLFLDKEYDECTSDGREDGRLWCATTYDYKADEKWGFCETEEEAAKRRQMQEAEMMYQTGMKILNGSNKKSQKREAYRYLQKAASMNHTKALERV Predict reactive species Full Name: sel-1 suppressor of lin-12-like (C. elegans) Calculated Molecular Weight: 89 kDa Observed Molecular Weight: 89 kDa GenBank Accession Number: NM_005065 Gene Symbol: SEL1L Gene ID (NCBI): 6400 RRID: AB_3671858 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9UBV2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.