Proteintech, 84424-4-RR, PSMA5 Recombinant monoclonal antibody

Size: 20ul / 100ul The PSMA5 (84424-4-RR) by Proteintech is a Recombinant antibody targeting PSMA5 in IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 84424-4-RR targets PSMA5 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: mouse liver tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, U2OS cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag34928 Product name: Recombinant human PSMA5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-241 aa of BC103751 Sequence: MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTSEGVCLAVEKRITSPLMEPSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHWFTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVIKDI Predict reactive species Full Name: proteasome (prosome, macropain) subunit, alpha type, 5 Calculated Molecular Weight: 241 aa, 26 kDa GenBank Accession Number: BC103751 Gene Symbol: PSMA5 Gene ID (NCBI): 5686 RRID: AB_3671951 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P28066 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.