{"product_id":"proteintech-84430-2-rr","title":"Proteintech, 84430-2-RR, SIRP Alpha\/CD172a Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe SIRP Alpha\/CD172a (84430-2-RR) by Proteintech is a Recombinant antibody targeting SIRP Alpha\/CD172a in WB, IHC, IF\/ICC, ELISA applications with reactivity to mouse samples\n84430-2-RR targets SIRP Alpha\/CD172a in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue, RAW 264.7 cells\nPositive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: RAW 264.7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a \"do not eat me\" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: FusionProtein Predict reactive species\nFull Name: signal-regulatory protein alpha\nCalculated Molecular Weight: 56 kDa\nObserved Molecular Weight: 90-140 kDa\nGenBank Accession Number: NM_007547.4\nGene Symbol: Sirpa\nGene ID (NCBI): 19261\nRRID: AB_3671957\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P97797-2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888443314345,"sku":"84430-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-84430-2-rr","provider":"Iright","version":"1.0","type":"link"}