Proteintech, 84472-4-RR, VPS28 Recombinant monoclonal antibody

Size: 20ul / 100ul The VPS28 (84472-4-RR) by Proteintech is a Recombinant antibody targeting VPS28 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 84472-4-RR targets VPS28 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, Jurkat cells, THP-1 cells, U-251 cells Positive IF/ICC detected in: A549 cells Positive FC (Intra) detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7784 Product name: Recombinant human VPS28 protein Source: e coli. -derived, PKG Tag: GST Domain: 1-221 aa of BC006485 Sequence: MFHGIPATPGIGAPGNKPELYEEVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAACSRLLVQYKAAFRQVQGSEISSIDEFCRKFRLDCPLAMERIKEDRPITIKDDKGNLNRCIADVVSLFITVMDKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRFLHA Predict reactive species Full Name: vacuolar protein sorting 28 homolog (S. cerevisiae) Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 28 kDa GenBank Accession Number: BC006485 Gene Symbol: VPS28 Gene ID (NCBI): 51160 RRID: AB_3671995 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9UK41 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.