Proteintech, 84500-1-RR, SELPLG/CD162 Recombinant monoclonal antibody

Size: 20ul / 100ul The SELPLG/CD162 (84500-1-RR) by Proteintech is a Recombinant antibody targeting SELPLG/CD162 in IHC, IF/ICC, ELISA applications with reactivity to human samples 84500-1-RR targets SELPLG/CD162 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Jurkat cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:4000-1:16000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant Protein Predict reactive species Full Name: selectin P ligand Calculated Molecular Weight: 412 aa, 43 kDa GenBank Accession Number: BC029782 Gene Symbol: SELPLG Gene ID (NCBI): 6404 RRID: AB_3672013 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q14242 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.