Proteintech, 84537-5-RR, RAD51C Recombinant monoclonal antibody

Size: 20ul / 100ul The RAD51C (84537-5-RR) by Proteintech is a Recombinant antibody targeting RAD51C in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 84537-5-RR targets RAD51C in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells, MDA-MB-231 cells, MCF-7 cells, T-47D cells, mouse testis tissue Positive IF/ICC detected in: HEK-293T cells Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag35070 Product name: Recombinant human RAD51C protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC107753 Sequence: MRGKTFRFEMQRDLVSFPLSPAVRVKLVSAGFQTAEELLEVKPSELSKEVGISKAEALETLQIIRRECLTNKPRYAGTSESHKKCTALELLEQEHTQGFIITFCSALDDILGGGVPLMKTTEICGAPGVGKTQLCMQLAVDVQIPECFGGVAGEAVFIDTEGSFMVDRVVDLATACIQHLQLIAEKHKGEEHRKALEDFTLDNILSHIYYFRCRDYTELLAQVYLLPDFLSEHSKVRLVIVDGIAFPFRHDLDDLSLRTRLLNGLAQQMISLANNHRLAVILTNQMTTKIDRNQALLVPALGESWGHAATIRLIFHWDRKQRLATLYKSPSQKECTVLFQIKPQGFRDTVVTSACSLQTEGSLSTRKRSRDPEEEL Predict reactive species Full Name: RAD51 homolog C (S. cerevisiae) Calculated Molecular Weight: 376 aa, 42 kDa Observed Molecular Weight: 40-42 kDa GenBank Accession Number: BC107753 Gene Symbol: RAD51C Gene ID (NCBI): 5889 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O43502 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.