{"product_id":"proteintech-84551-1-rr","title":"Proteintech, 84551-1-RR, Acetyl-Histone H2B (Lys120) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Acetyl-Histone H2B (Lys120) (84551-1-RR) by Proteintech is a Recombinant antibody targeting Acetyl-Histone H2B (Lys120) in WB, IF\/ICC, Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human, mouse samples\n84551-1-RR targets Acetyl-Histone H2B (Lys120) in WB, IF\/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Trichostatin A treated NIH\/3T3 cells\nPositive IF\/ICC detected in: Trichostatin A treated HeLa cells\nPositive Dot Blot detected in: peptide\nPositive ChIP-qPCR detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\nDOT BLOT: DOT BLOT : 1:10-1:100\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHistones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome\/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: histone cluster 2, H2be\nCalculated Molecular Weight: 14 kDa\nObserved Molecular Weight: 25 kDa\nGenBank Accession Number: BC005827\nGene Symbol: Histone H2B\nGene ID (NCBI): 8349\nRRID: AB_3672059\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: Q16778\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888687337641,"sku":"84551-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-84551-1-rr","provider":"Iright","version":"1.0","type":"link"}