{"product_id":"proteintech-84564-6-rr","title":"Proteintech, 84564-6-RR, Phospho-Estrogen Receptor α (Ser118) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-Estrogen Receptor α (Ser118) (84564-6-RR) by Proteintech is a Recombinant antibody targeting Phospho-Estrogen Receptor α (Ser118) in WB, FC (Intra), ELISA applications with reactivity to human samples\n84564-6-RR targets Phospho-Estrogen Receptor α (Ser118) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: T-47D cells\nPositive FC (Intra) detected in: λ phosphatase treated MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEstrogen Receptor α (ERα, gene symbol ESR1) is a member of the nuclear-receptor superfamily that functions as a ligand-dependent transcription factor. Dependent upon cell and promoter context, ERα regulates transcription through its two activation domains, AF-1 and AF-2. AF-2 is contained within the LBD and becomes active upon E2 binding. AF-1 however, is activated by phosphorylation of ERα at several sites, most notably serine 118 (S118) and serine 167 (S167), sites that are regulated by multiple signaling pathways. (PMID: 25597633)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: estrogen receptor 1\nCalculated Molecular Weight: 595 aa, 66 kDa\nObserved Molecular Weight: 66 kDa\nGenBank Accession Number: BC128573\nGene Symbol: ER\nGene ID (NCBI): 2099\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P03372\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888655945897,"sku":"84564-6-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-84564-6-rr","provider":"Iright","version":"1.0","type":"link"}