Proteintech, 84640-1-RR, SPHK1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SPHK1 (84640-1-RR) by Proteintech is a Recombinant antibody targeting SPHK1 in WB, ELISA applications with reactivity to human samples 84640-1-RR targets SPHK1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: THP-1 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: sphingosine kinase 1 Calculated Molecular Weight: 51 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: NM_182965 Gene Symbol: SPHK1 Gene ID (NCBI): 8877 RRID: AB_3672099 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9NYA1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.