{"product_id":"proteintech-84650-4-rr","title":"Proteintech, 84650-4-RR, ARNT\/HIF-1 beta Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe ARNT\/HIF-1 beta (84650-4-RR) by Proteintech is a Recombinant antibody targeting ARNT\/HIF-1 beta in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, rat samples\n84650-4-RR targets ARNT\/HIF-1 beta in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, HSC-T6 cells,  HeLa cells,  HepG2 cells\nPositive IF\/ICC detected in: HepG2 cells\nPositive FC (Intra) detected in: HeLa cells, HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1\/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1\/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag5507 Product name: Recombinant human ARNT,HIF1B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-348 aa of BC060838 Sequence: MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQQQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSS Predict reactive species\nFull Name: aryl hydrocarbon receptor nuclear translocator\nCalculated Molecular Weight: 416 aa, 46 kDa, 87 kDa\nObserved Molecular Weight: 87 kDa\nGenBank Accession Number: BC060838\nGene Symbol: ARNT\/HIF-1 beta\nGene ID (NCBI): 405\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P27540\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888708473001,"sku":"84650-4-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-84650-4-rr","provider":"Iright","version":"1.0","type":"link"}