Proteintech, 84657-4-RR, PSPC1 Recombinant monoclonal antibody

Size: 20ul / 100ul The PSPC1 (84657-4-RR) by Proteintech is a Recombinant antibody targeting PSPC1 in WB, ELISA applications with reactivity to human samples 84657-4-RR targets PSPC1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, PC-3 cells, MDA-MB-453 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information PSPC1 (paraspeckle component 1), a known paraspeckle biomarker, is a putative transcription factor that belongs to the Drosophila behavior/human splicing (DBHS) family. PSPC1 was recently identified as a contextual determinant of tumor progression in multiple cancer types involving oncogenic reprogramming to switch proapoptotic TGF-β to prometastatic TGF-β via hijacking of Smad2/3 targeting. Upregulated PSPC1 is correlated with advanced tumor stages and poor survival of patients with breast, lung, and liver cancers. Upregulated PSPC1 potentiates expression of mesenchymal markers, EMT transcription factors (EMT-TF), cancer stem-like cell transcription factors (CSC-TF), and c-Myc-related proliferation genes, thus promoting migration, invasion, spheroid formation, tumor formation, and metastasis. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag10136 Product name: Recombinant human PSPC1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC014184 Sequence: MMLRGNLKQVRIEKNPARLRALESAVGESEPAAAAAMALALAGEPAPPAPAPPEDHPDEEMGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLR Predict reactive species Full Name: paraspeckle component 1 Calculated Molecular Weight: 523 aa, 59 kDa Observed Molecular Weight: 66 kDa GenBank Accession Number: BC014184 Gene Symbol: PSPC1 Gene ID (NCBI): 55269 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q8WXF1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.