Proteintech, 84716-2-RR, PRDM8 Recombinant monoclonal antibody

Size: 20ul / 100ul The PRDM8 (84716-2-RR) by Proteintech is a Recombinant antibody targeting PRDM8 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse samples 84716-2-RR targets PRDM8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: SH-SY5Y cells Positive IHC detected in: mouse eye tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse eye tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information PRDI-BF1 and RIZ homology domain zinc fnger protein 8 (PRDM8) belongs to the PRDM family characterized by a conserved N-terminal PR domain related to the catalytic SET domains. PRDM8 is a key regulator in neural development and testis steroidogenesis (PMID: 32819411). PRDM8 is mainly demonstrated as a crucial regulatory molecule during neural development and testis steroidogen and expressed at low levels in HCC tissues (PMID: 29572888). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag23373 Product name: Recombinant human PRDM8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC071584 Sequence: MEDTGIQRGIWDGDAKAVQQCLTDIFTSVYTTCDIPENAIFGPCVLSHTSLYDSIAFIALKSTDKRTVPYIFRVDTSAANGSSEGLMWLRLVQSARDKEEQNLEAYIKNGQLFYRSLRRIAKDEELLVWYGKELTELLLLCPSRSHNKMNGSSPYTCLECSQRFQFEFPYVAHLRFRCPKRLHSADISPQDEQGGGVGTKDHGGGGGGGKDQQQQQQEAPLGPGPKFCKAGPLHHYPSPSPESSNPSAAAGGSSAKPSTDFHNLARELENSRGGSSCSPAQSLSSGSGSGGGGGHQEAELSPDGIATGGGKGKRKFPEEAAEGGGGAGLVGGRGRFVERPLPASKEDLVC Predict reactive species Full Name: PR domain containing 8 Calculated Molecular Weight: 689 aa, 72 kDa Observed Molecular Weight: 72 kDa GenBank Accession Number: BC071584 Gene Symbol: PRDM8 Gene ID (NCBI): 56978 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9NQV8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.