{"product_id":"proteintech-84834-1-rr","title":"Proteintech, 84834-1-RR, Phospho-POLR2A (Ser5) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-POLR2A (Ser5) (84834-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-POLR2A (Ser5) in WB, IF\/ICC, FC (Intra), ELISA, ChIP-qPCR applications with reactivity to human samples\n84834-1-RR targets Phospho-POLR2A (Ser5) in WB, IF\/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, λ phosphatase treated HeLa cells\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: λ phosphatase treated HeLa cells\nPositive ChIP-qPCR detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRNA Pol II consists of 12 subunits and is responsible for the expression of mRNA and lincRNA genes within cells. The subunits of RNA polymerases have been shown to play specific roles in Pol II CTD dephosphorylation, tissue-specific transcription, mRNA decay, and translation. Pol II transcription involves initiation through recruitment and CTD serine 5 phosphorylation, elongation with CTD serine 2 phosphorylation, and termination. (PMID: 38842922)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa\nCalculated Molecular Weight: 1970 aa, 217 kDa\nObserved Molecular Weight: 270 kDa\nGenBank Accession Number: BC137231\nGene Symbol: POLR2A\nGene ID (NCBI): 5430\nRRID: AB_3672142\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P24928\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888358969513,"sku":"84834-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-84834-1-rr","provider":"Iright","version":"1.0","type":"link"}