{"product_id":"proteintech-84839-1-rr","title":"Proteintech, 84839-1-RR, Phospho-EGFR (Tyr869) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-EGFR (Tyr869) (84839-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-EGFR (Tyr869) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n84839-1-RR targets Phospho-EGFR (Tyr869) in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: EGF treated A431 cells\nPositive IF\/ICC detected in: EGF treated A431 cells\nPositive FC (Intra) detected in: EGF treated A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEpidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase (RTK) family and plays a critical role in a wide variety of cellular functions, including proliferation, differentiation and apoptosis. After EGFR homodimerization with extracellular ligands such as epidermal growth factor (EGF), the intracellular tyrosine kinase domain is activated and autophosphorylates multiple tyrosine residues in the C‐terminal tail domain. (PMID: 23822636) Some scientists call EGFR Tyr869 as EGFR Tyr845, the site where the signaling peptide is removed.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)\nCalculated Molecular Weight: 1210 aa, 134 kDa\nObserved Molecular Weight: 180 kDa\nGenBank Accession Number: BC094761\nGene Symbol: EGFR\nGene ID (NCBI): 1956\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P00533\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888801009833,"sku":"84839-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-84839-1-rr","provider":"Iright","version":"1.0","type":"link"}