Proteintech, 84894-5-RR, PMS2 Recombinant monoclonal antibody

Size: 20ul / 100ul The PMS2 (84894-5-RR) by Proteintech is a Recombinant antibody targeting PMS2 in WB, IHC, FC (Intra), ELISA applications with reactivity to human samples 84894-5-RR targets PMS2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293T cells, PC-3 cells, MCF-7 cells, A431 cells, Caco-2 cells, Positive IHC detected in: human tonsillitis tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag33508 Product name: Recombinant human PMS2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 700-862 aa of BC093921 Sequence: QHATDEKYNFEMLQQHTVLQGQRLIAPQTLNLTAVNEAVLIENLEIFRKNGFDFVIDENAPVTERAKLISLPTSKNWTFGPQDVDELIFMLSDSPGVMCRPSRVKQMFASRACRKSVMIGTALNTSEMKKLITHMGEMDHPWNCPHGRPTMRHIANLGVISQN* Predict reactive species Full Name: PMS2 postmeiotic segregation increased 2 (S. cerevisiae) Calculated Molecular Weight: 862 aa, 96 kDa Observed Molecular Weight: 117 kDa GenBank Accession Number: BC093921 Gene Symbol: PMS2 Gene ID (NCBI): 5395 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P54278 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.