{"product_id":"proteintech-84906-1-rr","title":"Proteintech, 84906-1-RR, Phospho-EGFR (Tyr1197) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-EGFR (Tyr1197) (84906-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-EGFR (Tyr1197) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n84906-1-RR targets Phospho-EGFR (Tyr1197) in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: EGF treated A431 cells\nPositive IF\/ICC detected in: EGF treated A431 cells\nPositive FC (Intra) detected in: EGF treated A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEpidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB\/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). The EGFR autophosphorylation sites Tyr-992 and Tyr-1173 (also known as Tyr 1197)  have been shown to play a critical role in the activation of the MAPK cascade following EGF stimulation. (PMID: 15708852) Some scientists call EGFR Tyr1197 as EGFR Tyr1173, the site where the signaling peptide is removed.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)\nCalculated Molecular Weight: 1210 aa, 134 kDa\nObserved Molecular Weight: 175 kDa\nGenBank Accession Number: BC094761\nGene Symbol: EGFR\nGene ID (NCBI): 1956\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P00533\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888534245545,"sku":"84906-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-84906-1-rr","provider":"Iright","version":"1.0","type":"link"}