Proteintech, 84947-4-RR, RPS3 Recombinant monoclonal antibody

Size: 20ul / 100ul The RPS3 (84947-4-RR) by Proteintech is a Recombinant antibody targeting RPS3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 84947-4-RR targets RPS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, COLO 320 cells, NIH/3T3 cells, mouse brain tissue, human brain tissue, Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information 40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7346 Product name: Recombinant human RPS3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-243 aa of BC003137 Sequence: MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPVPTA Predict reactive species Full Name: ribosomal protein S3 Calculated Molecular Weight: 27 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC003137 Gene Symbol: RPS3 Gene ID (NCBI): 6188 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P23396 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.