{"product_id":"proteintech-84956-4-rr","title":"Proteintech, 84956-4-RR, RCN2 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe RCN2 (84956-4-RR) by Proteintech is a Recombinant antibody targeting RCN2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n84956-4-RR targets RCN2 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: rat liver tissue, HeLa cells,  HEK-293 cells,  U-251 cells,  mouse liver tissue\nPositive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells, HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:400-1:1600\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nReticulocalbin-2 (RCN2) is also named as endoplasmic reticulum Ca2+-binding protein of 55 kDa (ERC55) and E6-binding protein (E6BP). It is strictly located in endoplasmic reticulum (PMID: 34094681). RCN2 is a low-affinity Ca2+ binding protein localized to the secretory pathway of mammalian cells,  belongs to a member of the EF-hand Ca2+ binding protein family, which participate in the secretory process, chaperone activity, signal transduction as well as participation in a large variety of disease processes (PMID: 19260022). As for cancer research, several studies showed that RCN2 was related to tumor progression, such as mammary cancer,  cervical cancer, neck squamous cell carcinoma and colorectal cancer (PMID: 16351756, PMID: 32422974). RCN2 played a pivotal role in HCC pathogenesis through regulating activation of the epidermal growth factor receptor  (EGFR) pathway and modulating epidermal growth factor receptor EGFR dimerization and internalization (PMID: 28745317).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag0247 Product name: Recombinant human RCN2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 24-209 aa of BC004892 Sequence: KAEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLE Predict reactive species\nFull Name: reticulocalbin 2, EF-hand calcium binding domain\nCalculated Molecular Weight: 37 kDa\nObserved Molecular Weight: 55 kDa\nGenBank Accession Number: BC004892\nGene Symbol: RCN2\nGene ID (NCBI): 5955\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q14257\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888814084265,"sku":"84956-4-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-84956-4-rr","provider":"Iright","version":"1.0","type":"link"}