{"product_id":"proteintech-84966-2-rr","title":"Proteintech, 84966-2-RR, Eg5 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Eg5 (84966-2-RR) by Proteintech is a Recombinant antibody targeting Eg5 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n84966-2-RR targets Eg5 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HepG2 cells,  HEK-293 cells\nPositive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag20001 Product name: Recombinant human KIF11 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 707-1052 aa of BC136474 Sequence: LTEDLKTIKQTHSQELCKLMNLWTERFCALEEKCENIQKPLSSVQENIQQKSKDIVNKMTFHSQKFCADSDGFSQELRNFNQEGTKLVEESVKHSDKLNGNLEKISQETEQRCESLNTRTVYFSEQWVSSLNEREQELHNLLEVVSQCCEASSSDITEKSDGRKAAHEKQHNIFLDQMTIDEDKLIAQNLELNETIKIGLTKLNCFLEQDLKLDIPTGTTPQRKSYLYPSTLVRTEPREHLLDQLKRKQPELLMMLNCSENNKEETIPDVDVEEAVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPLRA Predict reactive species\nFull Name: kinesin family member 11\nCalculated Molecular Weight: 1056 aa, 119 kDa\nObserved Molecular Weight: 120-130 kDa\nGenBank Accession Number: BC136474\nGene Symbol: Eg5\nGene ID (NCBI): 3832\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P52732\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888739209385,"sku":"84966-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-84966-2-rr","provider":"Iright","version":"1.0","type":"link"}