Proteintech, 84986-3-RR, TRPA1 Recombinant monoclonal antibody

Size: 20ul / 100ul The TRPA1 (84986-3-RR) by Proteintech is a Recombinant antibody targeting TRPA1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 84986-3-RR targets TRPA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, SH-SY5Y cells, U2OS cells, mouse brain tissue, rat brain tissue Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information TRPA1, also named as ANKTM1, belongs to the transient receptor family. TRPA1 is a receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. TRPA1 has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. It acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. It may be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: transient receptor potential cation channel, subfamily A, member 1 Calculated Molecular Weight: 140 kDa Observed Molecular Weight: 130 kDa GenBank Accession Number: NM_007332 Gene Symbol: TRPA1 Gene ID (NCBI): 8989 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O75762 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.