Proteintech, 85024-6-RR, RECQL Recombinant monoclonal antibody

Size: 20ul / 100ul The RECQL (85024-6-RR) by Proteintech is a Recombinant antibody targeting RECQL in IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 85024-6-RR targets RECQL in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: U-251 cells Positive FC (Intra) detected in: Ramos cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information RECQL, also known as RecQ-like helicase, is a member of the RecQ DNA helicase family. These enzymes play a crucial role in maintaining genomic integrity by participating in various DNA repair processes, including mismatch repair, nucleotide excision repair, and direct repair. It's reported that the low RECQL expression is strongly associated with poor prognosis in breast cancer. RECQL expression may be a useful marker in estimating the prognosis of breast cancer patients. (PMID: 29914420) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6834 Product name: Recombinant human RECQL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 101-448 aa of BC001052 Sequence: NVTMAGKEVFLVMPTGGGKSLCYQLPALCSDGFTLVICPLISLMEDQLMVLKQLGISATMLNASSSKEHVKWVHAEMVNKNSELKLIYVTPEKIAKSKMFMSRLEKAYEARRFTRIAVDEVHCCSQWGHDFRPDYKALGILKRQFPNASLIGLTATATNHVLTDAQKILCIEKCFTFTASFNRPNLYYEVRQKPSNTEDFIEDIVKLINGRYKGQSGIIYCFSQKDSEQVTVSLQNLGIHAGAYHANLEPEDKTTVHRKWSANEIQVVVATVAFGMGIDKPDVRFVIHHSMSKSMENYYQESGRAGRDDMKADCILYYGFGDIFRISSMVVMENVGQQKLYEMVSYCQ Predict reactive species Full Name: RecQ protein-like (DNA helicase Q1-like) Calculated Molecular Weight: 73 kDa GenBank Accession Number: BC001052 Gene Symbol: RECQL Gene ID (NCBI): 5965 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P46063 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.