Proteintech, 85052-4-RR, TIGAR Recombinant monoclonal antibody

Size: 20ul / 100ul The TIGAR (85052-4-RR) by Proteintech is a Recombinant antibody targeting TIGAR in WB, IHC, ELISA applications with reactivity to human samples 85052-4-RR targets TIGAR in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, HEK-293 cells, HCT 116 cells, Jurkta cells, HeLa cells Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information The TP53-induced glycolysis and apoptosis regulator (TIGAR), first reported in 2006, is a downstream target gene of p53 and an important factor involved in glycolysis and apoptosis . It is indispensable in metabolism and is involved in metabolic syndrome, including hyperglycemia, insulin resistance, alcoholic fatty liver and tissue ischemia. TIGAR is highly expressed in many cancer cells to promote cell survival. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag17532 Product name: Recombinant human TIGAR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-270 aa of BC012340 Sequence: MARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR Predict reactive species Full Name: chromosome 12 open reading frame 5 Calculated Molecular Weight: 270 aa, 30 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC012340 Gene Symbol: TIGAR Gene ID (NCBI): 57103 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9NQ88 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.