{"product_id":"proteintech-85053-6-rr","title":"Proteintech, 85053-6-RR, Phospho-ErbB3\/HER3 (Tyr1289) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-ErbB3\/HER3 (Tyr1289) (85053-6-RR) by Proteintech is a Recombinant antibody targeting Phospho-ErbB3\/HER3 (Tyr1289) in WB, ELISA applications with reactivity to human samples\n85053-6-RR targets Phospho-ErbB3\/HER3 (Tyr1289) in WB, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF-7 cells, human Neuregulin-1 treated MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nV-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ErbB-3, HER3) is a member of the EGF receptor tyrosine kinase family including EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4) that are frequently overexpressed in a variety of carcinomas. Unlike EGFR and HER2, their paralogue HER3 does not possess intrinsic kinase activity due to substitutions at critical positions in the kinase domain. To phosphorylate tyrosine residues in the HER3 cytoplasmic tail to recruit and activate intracellular signaling molecules, it must interact with another functional tyrosine kinase. HER3 exhibits this crosstalk preferentially with HER2 and EGFR, with which it can form heterodimers. Ligand-induced hetero-dimerization with EGFR or HER2 promotes HER3-tyrosine phosphorylation at 6 positions (1054, 1197, 1222, 1260, 1276, and 1289) in the C-terminal tail capable of recruiting of the SH2-domain-containing p85 activator of phosphatidyl-inositol-3 kinase (PI3K) and promoting activation of the downstream Akt signaling pathway. (PMID: 35249128)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)\nCalculated Molecular Weight: 148 kDa\nObserved Molecular Weight: 185 kDa\nGenBank Accession Number: BC002706\nGene Symbol: ERBB3\nGene ID (NCBI): 2065\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P21860\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888801271977,"sku":"85053-6-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-85053-6-rr","provider":"Iright","version":"1.0","type":"link"}