Proteintech, 85126-4-RR, PON2 Recombinant monoclonal antibody

Size: 20ul / 100ul The PON2 (85126-4-RR) by Proteintech is a Recombinant antibody targeting PON2 in WB, ELISA applications with reactivity to mouse, rat samples 85126-4-RR targets PON2 in WB, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, L-929 cells, RAW 264.7 cells, mouse lung tissue, rat lung tissue, rat heart tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information PON2 (Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197). Specification Tested Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg2741 Product name: Recombinant Mouse Pon2 protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 30-354 aa of NM_183308.2 Sequence: ASREVESVDLPNCHLIKGIETGAEDIDILPNGLAFFSVGLKFPGLHSFAPDKPGGILMMDLKDERPRALELRVSWGFDLASFNPHGISTFIDDDDTVYLFVVNHPQFKSTVEIFKFQEEENSLLHLKTIKHELLPSVNDIIAVGPTHFYATNDHYFSDPFLKYLETYLNLHWANVVYYSPEEVKLVAEGFDSANGINISPDKKYVYVADILAHEIHVLEKQPNMNLTQLKVLQLGTLVDNLSIDPSSGDIWVGCHPNGQRLFVYHPNHPPASEVLRIQNILSEKPSVTTVYINNGSVLQGSSVATIYDRKLLVGTLYQKALYCEL Predict reactive species Full Name: paraoxonase 2 Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: NM_183308.2 Gene Symbol: Pon2 Gene ID (NCBI): 330260 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q62086 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.