{"product_id":"proteintech-85186-3-rr","title":"Proteintech, 85186-3-RR, MAFF Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe MAFF (85186-3-RR) by Proteintech is a Recombinant antibody targeting MAFF in WB, IHC, IF\/ICC, ELISA, ChIP-qPCR applications with reactivity to human, mouse samples\n85186-3-RR targets MAFF in WB, IHC, IF\/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: K-562 cells, mouse placenta tissue,  HuH-7 cells,  L02 cells,  4T1 cells\nPositive IHC detected in: human lung cancer tissue, human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: U2OS cells\nPositive ChIP-qPCR detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag3460 Product name: Recombinant human MAFF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-164 aa of BC015037 Sequence: MSVDPLSSKALKIKRELSENTPHLSDEALMGLSVRELNRHLRGLSAEEVTRLKQRRRTLKNRGYAASCRVKRVCQKEELQKQKSELEREVDKLARENAAMRLELDALRGKCEALQGFARSVAAARGPATLVAPASVITIVKSTPGSGSGPAHGPDPAHGPASCS Predict reactive species\nFull Name: v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)\nCalculated Molecular Weight: 17 aa, 8 kDa\nObserved Molecular Weight: 18-23 kDa\nGenBank Accession Number: BC015037\nGene Symbol: MAFF\nGene ID (NCBI): 23764\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9ULX9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888779219113,"sku":"85186-3-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-85186-3-rr","provider":"Iright","version":"1.0","type":"link"}