{"product_id":"proteintech-85188-4-rr","title":"Proteintech, 85188-4-RR, NSMCE2 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe NSMCE2 (85188-4-RR) by Proteintech is a Recombinant antibody targeting NSMCE2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n85188-4-RR targets NSMCE2 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, Jurkat cells,  K-562 cells,  A549 cells,  U2OS cells\nPositive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: U2OS cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNSMCE2 (E3 SUMO-protein ligase NSE2) has a C-terminal Siz\/PIAS-RING (SP-RING) domain with E3 SUMO ligase activity (PMID: 15601841). NSMCE2, an essential SUMO ligase of the SMC5\/6 complex suppresses cancer and aging in mice (PMID: 26443207).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag4545 Product name: Recombinant human NSMCE2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-247 aa of BC032797 Sequence: MPGRSSSNSGSTGFISFSGVESALSSLKNFQACINSGMDTASSVALDLVESQTEVSSEYSMDKAMVEFATLDRQLNHYVKAVQSTINHVKEERPEKIPDLKLLVEKKFLALQSKNSDADFQNNEKFVQFKQQLKELKKQCGLQADREADGTEGVDEDIIVTQSQTNFTCPITKEEMKKPVKNKVCGHTYEEDAIVRMIESRQKRKKKAYCPQIGCSHTDIRKSDLIQDEALRRAIENHNKKRHRHSE Predict reactive species\nFull Name: non-SMC element 2, MMS21 homolog (S. cerevisiae)\nCalculated Molecular Weight: 247 aa, 28 kDa\nObserved Molecular Weight: 28-32 kDa\nGenBank Accession Number: BC032797\nGene Symbol: NSMCE2\nGene ID (NCBI): 286053\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q96MF7\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888792916137,"sku":"85188-4-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-85188-4-rr","provider":"Iright","version":"1.0","type":"link"}