{"product_id":"proteintech-85238-1-rr","title":"Proteintech, 85238-1-RR, Phospho-IRS1 (Ser307) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-IRS1 (Ser307) (85238-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-IRS1 (Ser307) in WB, ELISA applications with reactivity to human, mouse samples\n85238-1-RR targets Phospho-IRS1 (Ser307) in WB, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: NIH\/3T3 cells, TNF alpha treated HeLa cells,  TNF alpha treated NIH\/3T3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSerine phosphorylation of insulin receptor substrate-1 (IRS-1) inhibits insulin signal transduction in a variety of cell backgrounds, which might contribute to peripheral insulin resistance. However, because of the large number of potential phosphorylation sites, the mechanism of inhibition has been difficult to determine. It has been confirmed that JNK and IKK are involved in the phosphorylation of IRS-1 (Ser307\/312). Additionally, activation of Akt, ERK, mTOR, and PKCζ correlates to IRS-1 phosphorylation at Ser307\/312, Ser267\/270, and Ser612\/616 in rodent\/human IRS-1. (PMID: 11606564, PMID: 12714600)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: insulin receptor substrate 1\nCalculated Molecular Weight: 1242 aa, 132 kDa\nObserved Molecular Weight: 180 kDa\nGenBank Accession Number: BC053895\nGene Symbol: IRS1\nGene ID (NCBI): 3667\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P35568\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888684454057,"sku":"85238-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-85238-1-rr","provider":"Iright","version":"1.0","type":"link"}