{"product_id":"proteintech-85246-1-rr","title":"Proteintech, 85246-1-RR, Phospho-Cyclin D1 (Thr286) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-Cyclin D1 (Thr286) (85246-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Cyclin D1 (Thr286) in WB, ELISA applications with reactivity to human, rat samples\n85246-1-RR targets Phospho-Cyclin D1 (Thr286) in WB, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MG132 treated HT-1080 cells, MG132 treated NIH\/3T3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCyclin D1 is an important regulator of G1-S phase cell cycle transition.  Active cyclin D1-cyclin dependent kinase 4\/6 complexes phosphorylate retinoblastoma protein, resulting in release of sequestered E2F transcription factors and subsequent expression of genes required for progression into S phase.  Cyclin D1 was localized to the nucleus and its rapid ubiquitin-dependent degradation shown to require phosphorylation at Thr286 by glycogen synthase kinase 3β (GSK3β). (PMID: 16504004)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: cyclin D1\nCalculated Molecular Weight: 295 aa, 34 kDa\nObserved Molecular Weight: 34 kDa\nGenBank Accession Number: BC000076\nGene Symbol: Cyclin D1\nGene ID (NCBI): 595\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P24385\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888800616617,"sku":"85246-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-85246-1-rr","provider":"Iright","version":"1.0","type":"link"}