{"product_id":"proteintech-85400-4-rr","title":"Proteintech, 85400-4-RR, VAMP8 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe VAMP8 (85400-4-RR) by Proteintech is a Recombinant antibody targeting VAMP8 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n85400-4-RR targets VAMP8 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: THP-1 cells, HL-60 cells,  A431 cells,  HeLa cells,  rat kidney tissue,  mouse kidney tissue\nPositive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: THP-1 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nVAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)\/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag7903 Product name: Recombinant human VAMP8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-81 aa of BC001634 Sequence: MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVLIC Predict reactive species\nFull Name: vesicle-associated membrane protein 8 (endobrevin)\nCalculated Molecular Weight: 11 kDa\nObserved Molecular Weight: 15 kDa\nGenBank Accession Number: BC001634\nGene Symbol: VAMP8\nGene ID (NCBI): 8673\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9BV40\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888522875049,"sku":"85400-4-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-85400-4-rr","provider":"Iright","version":"1.0","type":"link"}