{"product_id":"proteintech-85703-1-rr","title":"Proteintech, 85703-1-RR, Phospho-ATG4B (Ser383) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-ATG4B (Ser383) (85703-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-ATG4B (Ser383) in WB, FC (Intra), ELISA applications with reactivity to human samples\n85703-1-RR targets Phospho-ATG4B (Ser383) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells\nPositive FC (Intra) detected in: λ phosphatase treated K-562 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nATG4B stimulates autophagy by promoting autophagosome formation through reversible modification of ATG8. In humans, microtubule-associated protein 1 light chain, LC3B, is the best-characterized ATG8 isoform, and ATG4B, but not three other ATG4 isoforms, displays a highly selective preference toward LC3B. MST4 phosphorylates ATG4B at serine residue 383, which stimulates ATG4B activity and increases autophagic flux. (PMID: 29232556, PMID: 36056541)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: ATG4 autophagy related 4 homolog B (S. cerevisiae)\nCalculated Molecular Weight: 44 kDa\nObserved Molecular Weight: 44-48 kDa\nGenBank Accession Number: BC000719\nGene Symbol: ATG4B\nGene ID (NCBI): 23192\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9Y4P1\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888799600809,"sku":"85703-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-85703-1-rr","provider":"Iright","version":"1.0","type":"link"}